Bio-rad ChromLab™ Software Bedienungsanleitung PDF herunterladen (Seite 129)

Priming and Purging the System

Installation Guide | 127

Priming and Purging the NGC Scout System

Priming the NGC Scout system is a manual procedure and involves priming the

buffer blending valve starting from the Q4 port to Q1. Purging the NGC Scout

system is an automated procedure and involves removing air from one or all buffer

lines as well as the system pumps. Both procedures require access to ChromLab.

Tip: You can perform the steps on the NGC touch screen.

Priming the NGC Scout System

Note: This section assumes that you have not introduced buffer into the lines.

If you have buffer in the lines and are purging the lines of small air bubbles,

proceed to Purging the NGC Scout System on page 129.

To prime the buffer blending valve

1. Immerse the inlet tubing for ports Q4–Q1 in their respective buffer containers.

(See To plumb the buffer blending valve on page 111 for more information.)

2. Set the fluidic scheme to either NGC Scout or NGC Scout Plus.

3. In the fluidic scheme, touch or click the System Pump module to open its

dialog box.

4. In the System Pump dialog box, select Priming in the Mode dropdown list.

By default, all ports are closed. The Closed radio button is selected.

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Bio-rad ChromLab™ Software Bedienungsanleitung Seite 129