Bio-rad BioLogic LP System Bedienungsanleitung

For Technical Support, Call Your Local Bio-Rad Office, or in the US,Call 1-800-4BIORAD (1-800-424-6723)BioLogic™LPStarter KitInstructionManualCatalog

2. Connect the fraction collector to the BioLogic LP system using systemcable 3 as shown in Figure 3. Note: Both ends of cable 3 are identical.a. Conn

a. An SV-3 solenoid valve is used with the Model 2110 fraction collector.b. Plug the SV-3 valve directly into the diverter valve receptacle onthe back

3. Install tubing in pump as shown above.4. PharMed tubing, 1.6 mm ID (furnished with the BioLogic LP system), isrecommended for use with the starter

Below are the recommended tubing lengths when installing the instrument:• SV-3 to Waste [31 inches (78 cm)]• Pump Outlet to MV-6 [22 inches (57 cm)]•

2. Connect the cartridge between the injector valve outlet and the UVmonitor flow cell, as shown in the system plumbing diagram (Fig. 5). 3. Install 5

b. Press the soft key for CALIBRATE.c. Enter the Conductivity Cell Constant displayed on the conductivityflow cell cable. 2. In Manual mode: b. Press

c. Select “BioFrac” as your fraction collector. If your BioLogic LP system does not list the BioFrac, select 2128. The BioFrac communicates with the B

In Manual mode: a. Press the PUMP soft key, then press FLOW. b. Set flow rate to 1.5 ml/minute, press OK. Press START. c. Press the UV soft key, press

Section 19Setting the AlarmThe alarm sounds after 3 minutes to remind you to turn the valve backto the load position. (Proper technique is to switch t

Fig. 7.Section 22Final Check Before Starting Method1. Press the MANUAL soft key, then pres the START soft key to start thepump.2. Make sure that “A” b

4006073D.qxp 8/17/2009 7:45 AM Page CVR2

e. Chart Recorder: After zeroing the UV monitor, use the thumbwheelsto set both pens near the bottom of the chart recorder paper. Setpaper speed (12 c

Example LP Data View ChromatogramExample of chromatogram when running the BioLogic LP starter kit.Section 24Advanced Users: Automatic SampleInjectionI

3. Enter a pump program as described above—you may wish to recallyour earlier method and edit.Step 1. Buffer “A”, step Length 1 minute, flow rate 1.5

Catalog # Description731-8338 BioLogic LP System with BioFrac Fraction Collectorand LP Data View Software, 110/120 V, includes systemcables 3 and 15,

CatalogNumber Product Information741-0018 BioFrac Prep-20 Preparative Rack741-0007 BioFrac Fraction Collector Fittings Kit, includes replacementfittin

Catalog # Description750-0268 BioLogic Rack Expansion Kit, includes 2 trays, 2 vertical bars, 16 sleeves 750-0261 BioLogic Rack Tray, includes 1 rack

Catalog # Description731-8233 Male Luer Plugs, 25731-8229 Female Luer T-Connector, 10732-8302 0.8 mm Barb T-Connector, recommended for minimaldead-vol

Legal NoticesTygon is a trademark of Norton Co. Windows and Windows 95, 98, ME, NT, 2000, and XP are trademarksof Microsoft Corp.234006073D.qxp 8/17/

Life ScienceGroup00-0000 0000 Sig 0308Bulletin 0000 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Australi

Table of ContentsSection 1 Introduction...1Section 2 Starter Kit Components ...

4006073D.qxp 8/17/2009 7:45 AM Page ii

Section 1IntroductionThis manual guides you through the installation of your new BioLogicLP chromatography system. You will perform an actual chromato

2Section 3Buffer and Sample PreparationBuffer “A”1. Empty the contents of the bottle labeled “Buffer A” into a 500 ml graduated cylinder. 2. Add degas

Section 4System Setup1. Assemble the rack as shown in Figure 1. Be sure to install the racktray drain fitting, and provide a drain tube in case of spi

Fig. 2.Section 5Connecting a Chart Recorder1. If you will be recording data with a Bio-Rad 1327 chart recorder (discontinued item), set all chart reco

b. System Cable 4 (Mini-DIN/Banana Plugs) connects betweenCOND CHART and the channel 2 banana plug receptacles on the 1327. The red banana plug connec