Bio-rad S3™ Cell Sorter Bedienungsanleitung PDF herunterladen (Seite 104)

94 | S3 and S3e Cell Sorters

Maintenance

The window will give an option of repeating the gimball adjustment process or skipping and

proceeding to droplet calibration. If the camera views show that the stream is centered and the

nozzle line is green, droplet calibration can be done.

27. Close nozzle door.

28. Perform droplet calibration and QC to complete the tip swap process (Figure 109–112).

Prior to droplet calibration, the Adjust Stream Position window appear as a ﬁnal check for

positioning of the stream (Figure 109). The pinhole image can also be accessed from this

window (Figure 110).

Figure 108 displays the stream image (left, center) and the nozzle camera image (right). The

bottom of the nozzle will be displayed as a double dashed green line.

26. Lower or raise the nozzle, using the three gimballs, until the orange line and the yellow line

meet and turn green.

The S3 Cell Sorter will again use the micromotors to align the stream to the pinhole and the

lasers. The three camera views will be shown again. The micromotors will bring the stream into

focus again. This second alignment process should complete the alignment.

Fig. 108. Nozzle swap wizard window.

Fig. 109. Adjust Stream Position window streams view.

1 2 ... 99 100 101 102 103 104 105 106 107 108 109 ... 131 132

Keine Kommentare

Bio-rad S3™ Cell Sorter Bedienungsanleitung Seite 104