Bio-rad Model EG-1 Econo™ Gradient Monitor Bedienungsanleitung PDF herunterladen (Seite 15)

Larger or smaller than Chart recorder input Check chart recorder

expected gradient voltage set incorrectly settings (See Sections

profile 4.2 and 5)

If using Gradient Re-calibrate the

mode, buffer A and gradient mode

B values not set

correctly

Ghost peaks appear in Charged sample No problem

gradient profile detected (e.g. protein)

Air bubbles passing Use degassed buffers.

through flow cell Check for loose plumb-

ing connections

Chart recorder Chart recorder is not Check connections (See

not responding connected properly Sections 3.2 and 4.2)

Full scale of recorder Set recorder range to

set too high 1 V

Recorder has bottomed Readjust pen position

out

Flow cell not Check flow cell

connected in-line plumbing

No power Faulty power Check power cable

connection connection

Blown fuse Check fuses (See

Appendix A)

Unit not switched on Check power on/off

switch on rear panel

Conductivity Dirty flow cell Clean flow cell (See

measurements with Section 6)

same buffer appear

to decrease over time

Problem Possible Cause Solution

M7318150H 2/27/1 2:06 PM Page 12

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Bio-rad Model EG-1 Econo™ Gradient Monitor Bedienungsanleitung Seite 15