Bio-rad Model EG-1 Econo™ Gradient Monitor Bedienungsanleitung PDF herunterladen (Seite 14)

Model EG-1 Econo Gradient Monitor Control Unit and Flow Cell:

The control unit may be left on continually (power consumption is neg-

ligible).

Although the cases are chemical resistant, spills and splashes may

cause precipitates to form on the component cases. Use a squirt bottle

and soapy water to wash down the outer cases of the instrument.

Flow Cell

Clean the outside surface of the flow cell as described above.

The flow cell should be cleaned periodically with 1 M NaOH at 1

ml/min for 5 min. (SDS, 1 M HCl, 1 M NaOH, EtOH, and acetone will

not harm the Model EG-1 Econo Gradient Monitor flow cell.)

When the flow cell is not in use, disconnect, and clean out dissolved

salts using a syringe filled with deionized water.

Store the flow cell by injecting a dilute solution (15-25%) of ethanol or

isopropanol into the cell to prevent microbial growth. Use the end caps

provided to seal the flow cell’s inlet and outlet ports. Always rinse the

flow cell with water prior to the introduction of aqueous buffers. To

prevent the formation of salt crystals and solute deposits, do not allow

the flow cell to dry out.

Section 8

Troubleshooting

Problem Possible Cause Solution

Baseline drift or Bubbles in flow cell Use degassed buffers.

noisy signal Check for loose plumb-

ing connections

Dirty flow cell Clean flow cell; insure

that buffers are free of

particulate material

(See Section 6)

Bubbles passing Use degassed buffers.

through flow cell Check for loose plumb-

ing connections

Column not Equilibrate and/or

equilibrated or dirty regenerate column

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Bio-rad Model EG-1 Econo™ Gradient Monitor Bedienungsanleitung Seite 14