Bio-rad Microplate Manager Software Bedienungsanleitung PDF herunterladen (Seite 73)

Appendix

Quantitative ELISA Assay Tutorial

A tutorial showing how to set up an ELISA Assay, including a standard curve and

equations to transform the absorbance data into percentage values of the maximum

bound well, is given here.

1. To set up the protocol, Choose File > Instrument Setup.

2. Select Endpoint Reading Mode and the desired wavelength.

3. Open sample data file (Percent Max Binding Assay) with its protocol &

template.

C:\Program Files\Bio-Rad\Microplate Manager 6\Sample

Data

Defining the Template

Click the Template icon in the Main

Toolbar.

Blanks are defined as #. The average of

these # wells will be subtracted from all

the wells.

NSB are non-specific binding wells.

MAX is for the maximum binding well.

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Bio-rad Microplate Manager Software Bedienungsanleitung Seite 73