Bio-rad Mini Trans-Blot® Cell Bedienungsanleitung PDF herunterladen (Seite 5)

Table of Contents

Assembly and Disassembly .......................................... i

Wash Cell Before Use ................................................... i

Warranty ........................................................................ i

Section 1 Introduction .................................................. 1

1.1 Speciﬁcations ................................................. 3

1.2 Safety Instructions .......................................... 4

Section 2 Mini Trans-Blot Cell Assembly and

Preparation for Transfer ............................................... 5

2.1 Mini Trans-Blot Cell Description and

Assembly of Parts .......................................... 5

2.2 Preparation for Blotting .................................. 6

2.3 Acidic Transfers ............................................. 9

Section 3 Transfer Conditions ....................................10

3.1 General Guide to Transfer Buffers and

Running Conditions.......................................10

3.2 Notes on Electrophoretic Transfer

Conditions ....................................................11

3.3 Buffer Formulation ........................................13

Section 4 Strategies for Optimizing

Electrophoretic Transfer ............................................. 15

4.1 Optimizing Protein Transfer ...........................15

4.2 Optimizing DNA and RNA Transfer................ 18

Section 5 Choice of Blotting Membranes.................. 19

5.1 Protein Blotting ............................................ 19

5.2 DNA and RNA Blotting Membranes ............. 20

Section 6 Troubleshooting Guide .............................. 22

6.1 Electrophoretic Transfer ............................... 22

Section 7 References ................................................. 27

Section 8 Product Information .................................. 29

1 2 3 4 5 6 7 8 9 10 ... 39 40

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Bio-rad Mini Trans-Blot® Cell Bedienungsanleitung Seite 5