Bio-rad Trans-Blot® Turbo™ Transfer System Bedienungsanleitung

Trans-Blot® Turbo™ Blotting SystemInstruction Manual Catalog Number170-4155

Trans-Blot Transfer PacksTraydimensions(LxW):18.0x14.5cmStackdimensions(LxW):Miniformat(7.0x8.5cm),midiformat(13.5x8.5cm),+t

Table of ContentsBio-Rad Technical Support

Section 1: IntroductionSemi-drywesternblottingisacommontechniqueinmanyresearchanddiagnosticlaboratories.Conventionalsemi-dryblottingpr

Unpacking and Setup InstructionsTheTrans-BlotTurbostarterkit,catalog#170-4155,includesthemainunit,twocassettes(basesandlids),blotroll

Important:Thisinstrumentisintendedforlaboratoryuseonly.ThisproductconformstotheclassAstandardsforelectromagneticemissionsintended

Section 2: Equipment and Reagents OverviewTheTrans-Blot®Turbo™instrumentandreagentsaredesignedtoprovidefast,efcient,andreproduciblewest

Acceptable Gel Combinations Unacceptable Gel Combinations1 2 1 2Upper bay A 1miniformatgel 2minior1midiformatgels1miniformatgel 2minior

Section 3: Trans-Blot ® Turbo™ System Transfer SetupTheTurboprotocol,whencombinedwithTrans-Blottransferpacks,provideshighlyefcienttransfe

Mini Transfer Pack (Single Mini Gel) SetupThepackagingfortheminitransferpackhasthetwoionreservoirstacksintheleftandrightwellsofthe

Thetransferpacksarevacuumsealed.Checkthatthemembranedoesnotliftawaywiththefoilpackaginglid.Ifnecessary,holdtheedgeofthemembr

a.c.b.b.a.d.Fig.9.Assemblingtheminiformattransferpack.Fig.10.Assemblingthemidiformattransferpack.a.Laytheionreservoirstackwithth

Oncethestacksarepositionedinthecassettebase,placethecassettelidonthebase.Thelidisreversible,butensurethattheelectricalcontact

3.4 Transfer Using the Turbo ProtocolThe TurbobuttonimmediatelyaccessestheMIXEDMWprogram.Itisdesignedforefcienttransferofawidevariet

Note:AruncanbepausedbypressingthecorrespondingA:STOPorB:STOPNavigationbuttonduringtherun.Theuserhasachoicetocontinuefromth

3.5 Transfer Using Preprogrammed ProtocolsAllstepsareidenticaltothoseinSections3.3and3.3.a,exceptforSection3.3,step2,whichisdescrib

Fig.17.Userprotocolsscreen.3.6 Transfer Using a New ProtocolAssemblyoftheblottingsandwichisdescribedinsections3.1and3.2.Thissectiond

3.7 Transfer Using Traditional Semi-Dry ConsumablesTypicalprocedureutilizingtheTrans-BlotTurbosystemandconventionalsemi-drywesternblotting

3.8 Optimizing Transfer ConditionsThefollowingtechniques,aloneorincombination,willincreasetransferefciency:•Usealow-percentagegelo

Section 4: AppendicesAppendix A. MaintenanceTheTrans-Blot® Turbo™systemrequireslittlemaintenance.AfteryounishusingtheTrans-BlotTurbosyst

Bio-Rad Technical SupportFor help and technical advice, please contact the Bio-Rad Technical Support department. In the United States, the Technical S

Symptom/Problem SuggestedCorrectionsPoorornoelectrophoretictransfer;excessiveproteinremainsinthegel(asdetectedbystainingthegelafter

Swirlsormissingbands;diffusetransfersPoorcontactbetweenthemembraneandthegel.Airbubbles(orexcessbufferwhenusingtraditionalsemi-dr

Overview of the Three Protocol ModesTURBO:1miniformator2miniformatgels,or1midiformatgelin7min;1Mini-PROTEAN® TGX™ 

LIST:Bio-Radpreprogrammedprotocolsoruser-denedprotocols TechSupport:1-800-424-6723•www.bio-rad.com21Trans-Blot®Tur

NEW:Usercaneditprotocolparametersandname,save,andruntheprotocol22 TechSupport:1-800-424-6723•www.bio-rad.comTrans-Blot® Turbo™ Blotti

Consumables and Related ProductsTrans-Blot Accessories170-4151Trans-Blot®Turbo™Cassette,single170-4152

ii TechSupport:1-800-424-6723•www.bio-rad.com

Life ScienceGroup Sig 121210020688 Rev F US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Au

Safety Warning LabelsWarning:DonotopenorattempttorepairtheTrans-Blot® Turbo™instrumentorcassettes.Doingsowillvoidyourwarrantiesand

iv TechSupport:1-800-424-6723•www.bio-rad.com

Safety ComplianceThisinstrumenthasbeentestedandfoundtobeincompliancewithallapplicablerequirementsofthefollowingsafetyandelectromag

SpecificationsTrans-Blot® Turbo™ InstrumentDimensions:(LxWxH)26.0x21.1x20.4cmWeight:8lbwithcassettes,4.5lbwithoutcassettesInputpow