Bio-rad Mini Trans-Blot® Cell Bedienungsanleitung PDF herunterladen (Seite 28)

22 Mini-Trans-Blot Electrophoretic Transfer Cell

Section 6

Troubleshooting Guide

6.1 Electrophoretic Transfer

Poor electrophoretic transfer (as detected by staining

the gel)—proteins

1. Transfer time is too short.

• Increase the transfer time

2. Power is too low.

• Always check the current at the beginning

of the run. The current may be too low for a

particular voltage setting. If the buffer is prepared

improperly, the conductivity may be too low, and

not enough power will be delivered to the cell.

See the power guidelines for speciﬁc applications

in Section 3

• Remake the buffer or increase the voltage

• Try the high intensity blotting option

3. Power supply circuit is inoperative, or an inappropriate

power supply was used.

• Check the fuse. Be sure the voltage and current

output of the power supply match the needs of

the blotting instrument

4. Transfer apparatus is assembled incorrectly, and the

proteins are moving in the wrong direction.

• The gel/membrane sandwich may be assembled

in the wrong order or the cassette is inserted in

the tank facing the opposite orientation. Check

the polarity of the connections to the power sup-

ply

• Use a pre-stained protein standard to assess

transfer efﬁciency after blotting

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Bio-rad Mini Trans-Blot® Cell Bedienungsanleitung Seite 28