Bio-rad Mini Trans-Blot® Cell Bedienungsanleitung

Mini Trans-Blot® Electrophoretic Transfer CellInstruction ManualCatalog numbers170-3930170-3935170-3989170-3836

4 Mini-Trans-Blot Electrophoretic Transfer Cell1.2 Safety InstructionsPower to the Mini Trans-Blot cell is supplied by an external DC voltage power

Mini-Trans-Blot Electrophoretic Transfer Cell 5 Section 2Mini Trans-Blot® Cell Assembly and Preparation for Transfer2.1 Mini Trans-Blot Cell Descri

6 Mini-Trans-Blot Electrophoretic Transfer Cell2.2 Preparation for BlottingStore the blue cooling unit in your laboratory freezer at –20°C until re

Mini-Trans-Blot Electrophoretic Transfer Cell 7 * Removing any air bubbles which may have formed is very important for good results. Use a glass tu

8 Mini-Trans-Blot Electrophoretic Transfer Cell6. Add the frozen blue cooling unit. Place in tank and fill to the “blotting” mark on the tank.7. A

Mini-Trans-Blot Electrophoretic Transfer Cell 9 8. Put on the lid, plug the cables into the power supply, and run the blot. Refer to Section 3 for

10 Mini-Trans-Blot Electrophoretic Transfer CellSection 3Transfer Conditions3.1 General Guide to Transfer Buffers and Running ConditionsTable 3.1 p

Mini-Trans-Blot Electrophoretic Transfer Cell 11 3.2 Notes on Electrophoretic Transfer ConditionsThese variables will change total resistance and t

12 Mini-Trans-Blot Electrophoretic Transfer CellThe gel holders may warp, and the transfer buffer may boil and evaporate (further increasing conduc

Mini-Trans-Blot Electrophoretic Transfer Cell 13 Voltage limitsDo not increase voltage settings beyond those indicated in Table 3.1. If overnight t

14 Mini-Trans-Blot Electrophoretic Transfer Cell10 mM NaHCO3, 3 mM NaCO3, 20% methanol, pH 9.9Mix 0.84 g NaHCO3 and 0.318 g NaCO3 in ddH2O, add 200

Mini-Trans-Blot Electrophoretic Transfer Cell 15 Section 4Strategies for Optimizing Electrophoretic Transfer4.1 Optimizing Protein TransferGenerall

16 Mini-Trans-Blot Electrophoretic Transfer CellReduce buffer strengthDilution of transfer buffer results in lower current at any given voltage. Th

Mini-Trans-Blot Electrophoretic Transfer Cell 17 Use of PVDF membrane for protein transfers eliminates the alcohol requirement, and constitutes a l

18 Mini-Trans-Blot Electrophoretic Transfer Cell4.2 Optimizing DNA and RNA TransferProblems with elution of nucleic acids can be solved by altering

Mini-Trans-Blot Electrophoretic Transfer Cell 19 Section 5Choice of Blotting Membranes5.1 Protein Blotting MembranesNitrocellulose MembraneNitrocel

20 Mini-Trans-Blot Electrophoretic Transfer CellGreater retention during sequencing manipulations enhances the likelihood of obtaining information

Mini-Trans-Blot Electrophoretic Transfer Cell 21 Table 5.1 Guide to Protein Blotting MembranesMembrane Pore Size BindingCapacity(μg/cm2)NotesNitroc

22 Mini-Trans-Blot Electrophoretic Transfer CellSection 6Troubleshooting Guide6.1 Electrophoretic TransferPoor electrophoretic transfer (as detecte

Mini-Trans-Blot Electrophoretic Transfer Cell 23 5. Charge-to-mass ratio is incorrect.• Try a more basic or acidic transfer buffer to increase pr

Mini-Trans-Blot Electrophoretic Transfer Cell i Assembly and DisassemblyTo insure best performance from the Mini Trans-Blot® electrophoretic transf

24 Mini-Trans-Blot Electrophoretic Transfer CellSwirls or missing bands; diffuse transfers1. Poor contact between the membrane and the gel. Air bu

Mini-Trans-Blot Electrophoretic Transfer Cell 25 4. The gel electrophoresis may be at fault.• Artifacts of electrophoresis may be produced by poo

26 Mini-Trans-Blot Electrophoretic Transfer Cell4. Proteins <15,000 daltons may show diminished binding to 0.45 μm nitrocellulose, or may be wa

Mini-Trans-Blot Electrophoretic Transfer Cell 27 Section 7References1. Southern, E. M., J. Mol. Biol., 98, 503 (1975).2. Alwine, J. C., Kemp, D.

28 Mini-Trans-Blot Electrophoretic Transfer Cell18. Burnette, W. N., Anal. Biochem., 112, 195 (1981).19. Legocki, R. P. and Verma, D. P. S., Anal

Mini-Trans-Blot Electrophoretic Transfer Cell 29 Section 7Product InformationCatalogNumber Product DescriptionMini Trans-Blot® Cell170-3930 Mini Tr

Life ScienceGroupSig 1213M1703930 Rev K US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austr

Table of ContentsAssembly and Disassembly ... iWash Cell Before Use ...

Mini-Trans-Blot Electrophoretic Transfer Cell 1 Section 1IntroductionBlotting was first performed by Southern in 1975 with the transfer of DNA from

2 Mini-Trans-Blot Electrophoretic Transfer CellThis short 4 cm electrode distance allows generation of higher driving forces to produce efficient pr

Mini-Trans-Blot Electrophoretic Transfer Cell 3 1.1 SpecificationsConstructionElectrode module Molded polysulfoneGel holder cassettes Molded polycar