Bio-rad BioLogic LP System Bedienungsanleitung Seite 18
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Figure 3 shows how the flow cell and the filters can be changed. To remove the flow cell, you will need to
remove the retaining clip from the bottom of the optics module and pull the luer connectors away from their
holders. Then loosen the thumbscrews and remove the flow cell. When inserting the replacement flow cell,
make sure the O-ring is securely placed on the flow cell. To change the filter, loosen the filter’s thumbscrew
and lift out and rotate the filter holder. The selected wavelength is indicated by the raised arrow on the bot-
tom of the case.
WARNING: To avoid exposure to UV radiation, turn off the UV lamp when changing filters and flow cells.
2.4 CONDUCTIVITY DETECTOR
The Conductivity Detector consists of a small, portable Conductivity Flow Cell and the control circuitry which
resides in the BioLogic LP Controller. This monitoring system provides a means to check equilibration con-
ditions and the formation of a salt gradient, for example, in ion-exchange chromatography and hydrophobic
interaction chromatography. This data helps in optimizing purification protocols and column cleaning proce-
dures.
Figure 4. Conductivity Flow Cell and Separate Holder
The Conductivity Flow Cell consists of the following:
• Flow cell with Inlet/Outlet ports: For connection of the tubing, using luer fittings. The flow cell allows
flow in either direction.
• Signal Cable: For connection to the back of the BioLogic LP Controller. Electrical power for the
Conductivity Flow Cell is drawn off the signal cable.
The flow cell can be plumbed immediately after the UV Optics Module’s flow cell or at any other point in the
flowpath. A separate holder for the Conductivity Flow Cell is mounted to the system rack. The swept vol-
ume in the cell is a nominal 2 µl.
Conductivity is measured in milli-Siemens (mS). For accurate measurement, the flow cell must be calibrat-
ed. Refer to Table 10.
Nine fixed Conductivity ranges, from 500 to 0.5 mS Full Scale (mSFS), are available. The user may choose
a “custom” range using the “Min/Max” function. Refer to Table 10.
11
Description of Components
- BIOLOGIC LP 1
- CHROMATOGRAPHY SYSTEM 1
- TABLE OF CONTENTS 2
- GETTING STARTED QUICKLY 6
- 1.0 INTRODUCTION 8
- 1.2 FEATURES 9
- 1.3 UNPACKING 10
- Table 1. (continued) 11
- 2.0 DESCRIPTION OF COMPONENTS 13
- Table 3 14
- Table 3. (continued) 15
- 2.3 UV DETECTOR 17
- 2.4 CONDUCTIVITY DETECTOR 18
- 2.5 BIO-RAD SYSTEM OPTIONS 19
- Threshold 20
- Next tube 2 20
- Draining 20
- 3.0 SYSTEM CONNECTIONS 24
- Figure 10. Rack Assembly 25
- System Connections 27
- 4.0 SYSTEM PLUMBING 29
- Figure 12. Luer Fittings 30
- PLATEN ADJUST 31
- LOCK-RING 31
- LUER FITTING 31
- 4.3 PLUMBING THE SYSTEM 33
- Figure 14. System Plumbing 34
- 4.4 PURGING THE SYSTEM 37
- 5.0 SYSTEM OPERATION 38
- Table 5. (continued) 39
- Front Panel Controls 39
- 5.2 MANUAL MODE 41
- System Operation 42
- Table 6. (continued) 43
- Manual Mode Operation: Pump 43
- Table 6. (continued) 44
- Table 6 45
- Table 7 45
- Table 7. (continued) 46
- Table 8 48
- Table 9 49
- Table 9. (continued) 50
- Table 10 51
- Table 10. (continued) 52
- Table 11 53
- Table 11. (continued) 54
- Table 12 55
- 5.3 PROGRAMMING MODE 56
- Table 13 58
- Table 13. (continued) 59
- Figure 22. List of Methods 59
- 5.3.2 Creating a New Method 60
- Table 15 63
- Table 15. (continued 64
- Table 16 65
- Program Mode’s Pump Table 65
- Table 16. (continued) 66
- Table 17 69
- Table 17. (continued) 70
- Table 18 71
- Table 19 72
- Table 20 74
- Table 20. (continued) 75
- Table 21 77
- Table 21. (continued) 78
- Table 22 80
- Program Mode’s Alarm Table 80
- Table 22. (continued) 81
- 5.3.7 Entering Method Names 82
- 5.4 RUN MODE 83
- 5.4.1 Starting a Run 83
- Table 24. (continued) 84
- Run Mode: Starting a Run 84
- 5.4.2 Run in Progress 86
- Table 24 87
- Table 25 88
- Run Mode: Run Hold 88
- Table 26 89
- Run Mode: Run Pause 89
- 6.1 CLEANING AND STORAGE 92
- 6.2 PUMP CALIBRATION 92
- 6.2.1 Nominal Calibration 93
- 6.2.2 User Calibration 93
- Figure 32. UV Optics Module 96
- 6.6 TROUBLESHOOTING 97
- APPENDIX A. SPECIFICATIONS 99
- WARRANTY INFORMATION 101
- ORDERING INFORMATION 102
- 4006072 Rev D 106
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