Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Bedienungsanleitung PDF herunterladen (Seite 17)

Section 4

Preparing a Cartridge

For Use

Bio-Scale Mini Macro-Prep High Q, Macro-Prep

High S, and Macro-Prep DEAE cartridges contain

20% ethanol (v/v) as the storage solution. The fully

hydrated support is ready to use after equilibrating

the cartridge in the buffer of choice. To perform

buffer exchange, connect the cartridge to a liquid

chromatography system or peristaltic pump and

condition it as instructed below:

1. Set pump flow rate to 3.0 ml/min (731 cm/hr)

for the 1 ml cartridge or 6.0 ml/min (288 cm/hr)

for the 5 ml cartridge.

2. Wash the cartridge with degassed low-salt

buffer for 2 min.

3. Wash the cartridge with degassed high-salt

buffer for 5 min.

10006400B.qxp 7/10/2006 3:48 PM Page 13

1 2 ... 12 13 14 15 16 17 18 19 20 21 22 ... 27 28

Keine Kommentare

Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Bedienungsanleitung Seite 17