Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Bedienungsanleitung PDF herunterladen (Seite 21)

Table 4. Common buffers for ion exchange

chromatography.

Type of Buffering Buffer Range

Cation Exchanger

Acetic acid 4.8–5.2

Citric acid 4.2–5.2

HEPES 7.6–8.2

Lactic acid 3.6–4.3

MES 5.5–6.7

MOPS 6.5–7.9

Phosphate 6.7–7.6

PIPES 6.1–7.5

Pivalic acid 4.7–5.4

TES 7.2–7.8

Tricine 7.8–8.9

Anion Exchanger

Bicine 7.6–9.0

Bis-Tris 5.8–7.2

Diethanolamine 8.4–8.8

Diethylamine 9.5–11.5

L-Histidine 5.5–6.0

Imidazole 6.6–7.1

Pyridine 4.9–5.6

Tricine 7.8–8.9

Triethanolamine 7.3–8.0

Tris 7.5–8.0

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Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Bedienungsanleitung Seite 21