Bio-rad Prepacked Gravity Flow Columns for Ion Exchange Bedienungsanleitung PDF herunterladen (Seite 8)

Section 7

Temperature Limits

Increases in temperature tend to decrease

resin selectivity, and can be used to decrease

elution times. Increased temperature also

increases exchange kinetics, resulting in

sharper resolution. However, some temperature

limits must be observed with ion exchange

resins. Table 3 gives the maximum tempera-

ture for each resin packing.

Table 3. Temperature Limits

Maximum

Resin Packing Temperature (°C)

AG 50 resin 150

AG 1 resin 150 (50 °C if converted

to OH

–

form)

AG 2 resin 150° (30 °C if converted

to OH

–

form)

Chelex 100 resin 75

Bio-Rex 70 resin 100

Section 8

Troubleshooting

1. Variation in color (lot-to-lot): Sometimes

the color of the resin may vary from lot to

lot. This will not affect the ion exchange

capacity of the resin.

2. Unusual odor: Amines will sometimes

cleave from anion exchange resin after pro-

longed storage. This is not unusual. They

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