Bio-rad ENrich™ High-Resolution Size Exclusion Columns Bedienungsanleitung PDF herunterladen (Seite 6)

2 ENrich SEC Instruction Manual

The ENrich support is compatible with aqueous solutions of 6 M

guanidine-HCl and 8 M urea. Detergents and organic solvents

such as methanol, ethanol, and isopropanol may also be used.

In some cases, alcohols and stability agents such as glycerol

may increase the column backpressure. Care should be taken

to maintain a ﬂow rate at which the backpressure is below the

maximum operating pressure.

Table 1. ENrich SEC media and column characteristics.

SEC 70 SEC 650

Linear separation range, Da

500–70, 000 500–650, 000

Column dimensions,

diameter x height, mm

10 x 300 10 x 300

Column volume, ml 24 24

Nominal particle size, µm 10 ± 2 10 ± 2

Recommended ﬂow rates, ml/min* 0.5–1.0 0.75–1.25

Maximum recommended ﬂow rates,

ml/min*

1.5 2.0

Maximum operating pressure 600 psi,

4.1 MPa, 41 bar

600 psi,

4.1 MPa, 41 bar

Recommended sample volume, µl

<250 <250

Efﬁciency, plates/m

>20,000 >20,000

Working pH range 2–12 2–12

Operating temperatures, °C 4–40 4–40

* At room temperature. Viscosity may increase at lower temperatures, which will reduce the

recommended ﬂow rates.

1.3 Connection to the NGC

™

and Other

Chromatography Systems

The ENrich columns are ﬁtted with 10-32 type female ﬁttings on either

end. Standard 10-32 ﬁttings can be used to plumb the column to a

Bio-Rad

NGC

™

chromatography system or another vendor’s systems.

Adaptors are available for connection to systems that use 1/4-28 ﬁttings

(catalog # 750-0564).

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