Bio-rad Aurum™ Total RNA Mini Kit Bedienungsanleitung PDF herunterladen (Seite 25)

Problem Possible Cause Recommended Solution

Total RNA prep Incorrect use of wash Add the appropriate

performs poorly solutions volume of 95–100%

in downstream ethanol to the wash

applications solutions before initial

use

RNA is degraded See problem “RNA

degradation”

Ethanol contamination Add 1–3 min to the

in prep (eluate volumes centrifugation time

>80 µl) after the final wash step

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Bio-rad Aurum™ Total RNA Mini Kit Bedienungsanleitung Seite 25